Radiation hybrid mapping of 63 previously unreported equine microsatellite loci.

Source/description: Horse genomic DNA was digested with the restriction enzyme MboI, size selected by gel electrophoresis for fragments between 200 and 1200 bp, and ligated into the BamHI site of the M13 phage vector. Clones containing a potential microsatellite were identified by screening the library with [P] 5¢ end-labelled oligo [dCA]16 and oligo [dGT]16 probes. The DNA was isolated from positive plaques and the inserts were sequenced using an ABI 3100 automated sequencer. Primer pairs for PCR amplification of the markers were developed using the PRIMER program (Version 0.5; M. J. Daly, S. E. Lincoln and E. S. Lander, unpublished). Sequence accession numbers, repeat motif, PCR primer pairs and product sizes based on the cloned sequences are provided in Table 1.